"Photograph" - what is it, definition of the term
The term designates a visual record created by exposing a photosensitive medium to light reflected from a scene, object, or subject, producing a fixed image that can be reproduced, stored, or displayed.
Detailed information
Macro imaging of tiny arthropods such as ticks, insects, lice, and fleas demands precise control of depth of field, illumination, and subject positioning. The resulting picture must capture fine anatomical details while minimizing distortion.
A suitable camera system combines a DSLR or mirrorless body with a dedicated macro lens offering a 1:1 reproduction ratio. Extension tubes or bellows increase magnification for the smallest specimens. A stable tripod prevents camera shake, and a remote shutter release eliminates manual pressure on the camera.
Lighting considerations include:
- Continuous LED panels or ring lights to provide even, shadow‑free illumination.
- Diffusers to soften harsh reflections on glossy exoskeletons.
- Polarizing filters to reduce specular highlights on wet or oily surfaces.
Focus technique:
- Use live view with magnification to verify critical focus on the eyes or mouthparts.
- Employ manual focus to fine‑tune the focal plane.
- Apply focus stacking when depth of field is insufficient; capture multiple frames at different focus distances and merge them in post‑processing software.
Subject handling:
- Secure specimens on a low‑reflectance stage using fine pins or a soft brush.
- Keep organisms alive only as long as necessary; euthanize according to ethical guidelines when required for detailed study.
- Maintain a clean environment to avoid contaminating the image with debris or dust.
Post‑processing steps: