Understanding the Importance of Tick Analysis
Why Analyze Ticks?
Identifying Pathogens
Ticks collected for pathogen detection must be sent to facilities equipped for molecular or serological testing. Laboratories capable of processing tick specimens include government health agencies, academic research centers, and commercial diagnostic providers. Submitting specimens to these entities enables reliable identification of bacteria, viruses, and protozoa carried by ticks.
- State or provincial public‑health laboratories: accept individual or pooled tick samples, perform PCR or culture assays, and report results to health authorities.
- Federal disease‑control programs (e.g., CDC tick testing initiative): provide nationwide submission portals, issue guidance on packaging, and return detailed pathogen lists.
- University‑affiliated entomology or microbiology labs: offer specialized testing for emerging agents, often collaborating on research projects.
- Commercial diagnostic companies: deliver rapid turnaround, standardized panels for common tick‑borne pathogens, and electronic result delivery.
- Wildlife and environmental agencies: collect ticks during surveillance, forward them to partner labs for routine screening.
Proper labeling, preservation in ethanol or dry ice, and inclusion of collection metadata (location, date, host) are required by all recipients. Choosing the appropriate submission channel depends on geographic location, desired turnaround time, and the specific pathogens of interest.
Guiding Treatment Decisions
Submitting ticks for laboratory examination provides the diagnostic foundation for selecting appropriate therapeutic regimens. Accurate pathogen identification distinguishes cases that require antimicrobial intervention from those that can be managed conservatively, thereby preventing unnecessary drug exposure and reducing resistance pressure.
Common destinations for tick specimens include:
- State or provincial public health laboratories, which offer free testing for mandated vector‑borne diseases.
- University entomology or microbiology departments that conduct specialized assays for emerging agents.
- Commercial diagnostic firms that provide rapid polymerase chain reaction or serology panels for a fee.
- Veterinary clinics equipped with in‑house identification services, useful for animal‑related exposures.
- Citizen‑science platforms such as online tick‑submission portals, which forward samples to accredited reference labs.
Laboratory reports deliver pathogen‑specific information—species, infection stage, and co‑infection status. Clinicians use these data to:
- Confirm the need for empiric antibiotics versus watchful waiting.
- Choose the most effective drug class (e.g., doxycycline for Borrelia, azithromycin for Rickettsia).
- Determine treatment duration based on pathogen virulence and patient risk factors.
- Arrange follow‑up testing to monitor therapeutic response.
Timely submission, proper preservation (cool, dry storage), and complete metadata (geographic location, date of bite, host species) enhance result reliability, ensuring that treatment decisions are grounded in precise, evidence‑based insights.
Contributing to Public Health Surveillance
Submitting tick specimens for laboratory examination supports disease monitoring and informs public‑health decisions. Agencies that accept samples include state health departments, which route collections to regional reference laboratories; the Centers for Disease Control and Prevention, which operates a national tick repository; university entomology or epidemiology programs that maintain diagnostic facilities; and accredited commercial diagnostic companies that offer fee‑for‑service testing.
Typical submission procedures require a properly labeled specimen, a completed request form, and a brief travel history. Samples should be placed in a sealed container with moist cotton or ethanol, depending on the laboratory’s instructions. Shipping must comply with biosafety regulations and include a temperature‑controlled package when viable ticks are needed for pathogen isolation.
- State health department laboratories (e.g., California Department of Public Health, Texas Department of State Health Services)
- CDC’s Division of Vector-Borne Diseases tick repository
- University research labs with vector‑borne disease expertise (e.g., University of Rhode Island, University of Texas Medical Branch)
- Certified commercial diagnostic providers (e.g., Quest Diagnostics, LabCorp)
Adhering to these channels ensures that tick data enter surveillance systems promptly, enhancing early detection of emerging infections.
Where to Submit Ticks for Analysis
Public Health Agencies and Laboratories
State Health Departments
State health departments operate designated programs that accept tick specimens for laboratory evaluation. Each department maintains a vector‑borne disease unit responsible for receiving, identifying, and testing submitted material.
To submit a tick, follow these steps:
- Locate the appropriate state health department website or call the vector‑borne disease hotline. Contact information is listed under “Tick Surveillance” or “Arthropod Testing.”
- Complete the required submission form, providing details such as collection date, location, host species, and any observed symptoms.
- Package the tick in a sealed container with a moist cotton ball or ethanol, according to the department’s specimen‑preservation guidelines.
- Ship the package via a trackable carrier or deliver it in person to the department’s laboratory facility. Include the completed form and a prepaid shipping label if offered.
State health departments typically forward specimens to state public health laboratories equipped with molecular and morphological testing capabilities. Results, including species identification and pathogen presence, are returned to the submitter within a standard reporting window of 7–14 days. Some departments charge a nominal fee for processing; others provide the service free of charge for public health surveillance purposes.
Additional resources:
- Online portal for electronic submission tracking.
- List of accepted pathogens (e.g., Borrelia burgdorferi, Anaplasma phagocytophilum, Rickettsia spp.).
- Guidelines for bulk submissions during outbreak investigations.
Utilizing state health department channels ensures that tick analyses contribute to regional surveillance data, supporting timely public health interventions.
Local Health Departments
Local health departments act as the most accessible agencies for submitting ticks to be examined. They receive specimens from residents, conduct species identification, and test for pathogens such as Borrelia burgdorferi and Anaplasma spp.
To initiate a submission, contact the department’s vector‑borne disease unit, obtain the required intake form, and follow the packaging instructions. Specimens must be placed in a sealed vial with ethanol or dry ice, labeled with collection date and location, and accompanied by a completed questionnaire.
Submitting ticks to a local health department yields several advantages: results are returned within a standard reporting period, data contribute to regional surveillance maps, and the department provides guidance on personal protection and treatment options.
Typical procedure:
- Locate the nearest health department via the state health website or a phone directory.
- Call the vector‑borne disease line or visit the online portal to request a submission kit.
- Complete the intake form, describing the bite incident and tick characteristics.
- Place the tick in the provided container, seal, and include the form.
- Deliver the package in person or use a prepaid courier service as instructed.
While state laboratories and private diagnostic companies also accept ticks, local health departments remain the primary point of contact for community‑level testing and public‑health response.
University-Affiliated Labs
University‑affiliated laboratories serve as primary venues for the examination of tick specimens collected in field studies or public‑health investigations. These facilities combine academic expertise with specialized equipment, enabling accurate species identification, pathogen detection, and genomic analysis.
Typical services include morphological keys, molecular assays (PCR, qPCR, metagenomics), and pathogen isolation. Researchers receive detailed reports outlining species, infection status, and recommendations for further study. Turn‑around times range from 48 hours for basic identification to two weeks for comprehensive sequencing.
Submission guidelines are uniform across most campuses: specimens must be placed in sealed, ethanol‑filled vials or frozen at –20 °C, accompanied by a completed data sheet specifying collection location, date, host information, and any preliminary observations. Samples are mailed or delivered to the designated mailroom, where they are logged and assigned a tracking number.
Examples of university‑linked labs that accept tick submissions:
- Department of Entomology, University of California, Davis – Vector‑Borne Disease Center
- Institute of Ecology, University of Toronto – Arthropod Research Unit
- School of Veterinary Medicine, Cornell University – Tick‑Pathogen Laboratory
- Department of Biology, University of Edinburgh – Parasite Surveillance Group
Contact information for each laboratory is available on the respective university website; most provide email addresses for the principal investigator or a dedicated submission portal.
Private Laboratories
Specialized Tick Testing Services
Specialized tick testing services accept specimens from a range of sources. Veterinary clinics, public health laboratories, and university research centers provide dedicated portals for sample submission. Commercial laboratories that focus on vector-borne disease diagnostics also maintain online forms and prepaid shipping kits.
Typical submission process includes:
- Completion of a detailed questionnaire describing collection method, host species, and geographic location.
- Packaging of ticks in a sealed container with desiccant or ethanol, following the provider’s biosafety guidelines.
- Use of a pre-addressed, prepaid label supplied by the testing service to ensure rapid delivery.
Major providers in the United States and Canada include:
- CDC Arbovirus Diagnostic Laboratory – accepts mailed specimens via a secure online portal.
- IDEXX Tick Testing – offers drop‑off locations at veterinary practices and a mail‑in option.
- Quest Diagnostics Vector‑Borne Pathogen Panel – provides a nationwide shipping network with tracking.
- Eurofins Scientific Tick Analysis – serves European customers through regional collection centers.
International options consist of government health agencies, such as the UK’s Public Health England, and private entities like the Australian Centre for Disease Preparedness, each offering country‑specific submission guidelines.
Choosing a specialized service ensures accurate identification, pathogen detection, and access to expert interpretation of results, which supports surveillance programs and informs clinical decision‑making.
Veterinary Diagnostic Laboratories
Veterinary diagnostic laboratories accept tick specimens for pathogen testing, species identification, and antimicrobial‑resistance profiling. These facilities operate under accredited standards (e.g., CAP, ISO 15189) and provide results to veterinarians, researchers, and public‑health agencies.
Typical submission routes include:
- Mail‑in service – sealed, ethanol‑preserved or frozen ticks placed in a labeled vial, accompanied by a completed request form and a prepaid shipping label.
- In‑person drop‑off – designated reception desks at university‑affiliated labs or state animal health laboratories, where staff verify specimen integrity and record collection data.
- Courier collection – scheduled pickup by a specialized biological‑sample courier, with temperature‑controlled packaging and chain‑of‑custody documentation.
Key requirements for each submission:
- Accurate metadata – collection date, geographic coordinates, host species, and life stage of the tick.
- Preservation method – 70 % ethanol for DNA/RNA extraction, dry ice or liquid nitrogen for live‑culture assays.
- Compliance with regulations – permits for interstate transport of arthropods, adherence to biosafety guidelines.
Major providers in the United States and Canada include:
- University of Minnesota Veterinary Diagnostic Laboratory
- Texas A&M College of Veterinary Medicine & Biomedical Sciences
- Cornell University College of Veterinary Medicine
- Canadian Food Inspection Agency National Centre for Animal Health
International options comprise the European Reference Laboratory for Tick‑Borne Diseases (France) and the Australian Animal Health Laboratory (Victoria). Each laboratory publishes detailed submission protocols on its website, ensuring consistent handling and reliable diagnostic outcomes.
Online Submission Options
Online platforms provide the most efficient route for delivering tick specimens to laboratories equipped for pathogen detection, morphological identification, and genetic sequencing. Users upload high‑resolution images, descriptive metadata, and, when required, physical samples through secure web portals that integrate with laboratory information management systems.
- Dedicated vector surveillance portals hosted by public health agencies (e.g., CDC’s Tick Surveillance System) accept digital submissions and forward specimens to accredited reference labs.
- Commercial diagnostic services (e.g., TickCheck, iPathogen) offer paid online intake forms, immediate receipt confirmation, and turnaround‑time guarantees.
- Academic research consortia maintain shared repositories (e.g., VectorBase) where contributors register samples, attach collection coordinates, and request analysis through integrated pipelines.
- Open‑source citizen‑science platforms (e.g., iNaturalist) allow upload of tick photographs and locality data; participating experts provide preliminary identification and route confirmed cases to specialized laboratories.
Each option requires adherence to data standards: species name, collection date, geographic coordinates, and, when applicable, preservation method. Selecting the appropriate service depends on the intended depth of analysis, budget constraints, and urgency of results.
General Practitioners and Veterinarians
Initial Assessment and Guidance
When a tick is discovered, the first step is a rapid visual appraisal. Identify the life stage, size, and attachment site; note the date of removal and any visible signs of engorgement. Record the geographic location with the highest possible precision, using GPS coordinates or a detailed description of the environment.
Preserve the specimen promptly. Place the tick in a sealed, airtight container with a small amount of breathable material to prevent desiccation. If possible, store at 4 °C; for longer periods, freeze at –20 °C. Accompany the sample with a brief data sheet that includes species‑level identification (if known), host information, and collection details.
Submission options include:
- State or provincial public health laboratories that process vector‑borne disease specimens.
- University entomology or vector‑biology departments offering diagnostic services.
- Commercial diagnostic companies specializing in arthropod analysis.
- National reference centers that maintain tick collections and provide molecular testing.
- Online portals that accept mailed specimens and supply pre‑paid packaging.
Each receiving facility requires a completed request form, a signed consent for analysis, and, when applicable, a fee schedule. Verify the preferred shipping method, as some laboratories mandate dry ice for molecular assays, while others accept refrigerated parcels.
Follow the guidance provided by the chosen laboratory: adhere to labeling conventions, include any required permits, and retain a copy of the submission record for future reference. This systematic approach ensures accurate identification, reliable testing, and timely public‑health reporting.
Referral to Specialized Labs
Referral to specialized laboratories provides the most reliable identification and pathogen testing for ticks collected by professionals or the public. These facilities possess accredited microscopy, molecular diagnostics, and expertise in tick taxonomy, ensuring accurate results that support disease surveillance and clinical decision‑making.
Typical destinations for tick specimens include:
- State or provincial public health laboratories, which often operate under national disease‑control agencies and offer free or low‑cost testing for residents.
- University or research institute laboratories with entomology departments, providing detailed species confirmation and advanced pathogen panels.
- Federal agencies such as the Centers for Disease Control and Prevention (CDC) or the United States Department of Agriculture (USDA), which accept submissions for national monitoring programs.
- Commercial diagnostic companies that specialize in arthropod analysis, offering rapid turnaround and online reporting for clinicians and researchers.
- Private veterinary or medical diagnostic labs that hold CLIA certification, allowing integration of results into patient records.
Submitting specimens to these centers typically requires a sealed container, detailed collection data (location, date, host), and a completed request form. Following the specific submission guidelines of the chosen laboratory maximizes the likelihood of accurate identification and timely receipt of results.
Preparing Ticks for Submission
Safe Removal Techniques
Proper Tools and Methods
Submitting tick specimens for laboratory analysis requires reliable collection devices, secure packaging, and clear documentation. Use fine‑tipped forceps or a sterile aspirator to detach the arthropod without damage. Place the tick in a labeled vial containing 70 % ethanol for preservation or in a dry, breathable container if live testing is intended. Include a worksheet that records collection date, geographic coordinates, host species, and any observed symptoms. Seal the container with tape and place it in a rigid outer box to prevent breakage during transport.
When choosing a receiving facility, prioritize accredited public health laboratories, university entomology departments, or commercial diagnostic services that specialize in vector‑borne disease testing. Verify that the chosen lab offers the specific assays required—polymerase chain reaction for Borrelia, ELISA for tick‑borne viruses, or microscopy for protozoan parasites. Confirm the submission deadline and any temperature constraints; most facilities request specimens within 48 hours of collection and storage at 4 °C for live samples.
Typical submission workflow:
- Collect and preserve the tick according to the method described above.
- Complete the data sheet with precise location and host information.
- Package the specimen in compliance with the receiving laboratory’s guidelines.
- Ship via overnight courier with a tracking number, or deliver in person to the lab’s drop‑off counter.
- Retain copies of all documentation for reference and future reporting.
Adhering to these tools and procedures ensures accurate identification, minimizes contamination risk, and facilitates timely diagnostic results.
Avoiding Crushing the Tick
When sending a tick to a diagnostic facility, preserve its integrity by avoiding any pressure that could damage the specimen. Handle the tick with tweezers or gloves, keep it upright, and place it in a small, breathable container such as a folded paper envelope or a vented plastic vial. Do not place the insect in a sealed bag, as moisture buildup may cause swelling and rupture.
Common destinations for tick analysis include:
- State or provincial public health laboratories that accept mailed specimens.
- University entomology departments with dedicated arthropod identification services.
- Commercial diagnostic companies offering online submission portals.
- Non‑governmental organizations that operate regional tick surveillance programs.
Before dispatch, label the container with collection date, location, host species, and any observable symptoms. Seal the outer package with cushioning material to prevent crushing during transit, and choose a courier that guarantees rapid delivery. Following these procedures ensures that the tick arrives intact, allowing accurate identification and testing.
Storage and Packaging
Appropriate Containers
When sending tick specimens for identification or pathogen testing, the container must protect the sample, prevent damage, and comply with transport regulations. Use a rigid, sealable container such as a small polypropylene tube or a screw‑cap microcentrifuge tube. The container should be sized to hold the tick without crowding, allowing the specimen to lie flat and be easily retrieved. For multiple ticks, separate compartments or a small, ventilated plastic box with a secure lid are acceptable.
Preservation medium influences the choice of container. If the tick is placed in 70 % ethanol, the container must be chemically resistant; polypropylene or glass vials with tight‑fitting caps are suitable. For live or unfixed specimens, a breathable container such as a ventilated plastic container with a moist cotton pad maintains humidity while preventing escape. In all cases, the container should be clearly labeled with collection date, location, and collector information.
Transport requirements dictate additional constraints. When mailing specimens, the container must fit within a secondary packaging that meets postal regulations for biological samples. A sealed primary tube placed inside a padded envelope or a small insulated box satisfies these rules. Include a biohazard label if required by the receiving laboratory.
Key points for selecting an appropriate container:
- Rigid, sealable, and chemically inert (polypropylene, glass)
- Sized to avoid crowding or crushing the tick
- Compatible with preservation medium (ethanol, moist environment)
- Allows secure labeling
- Fits within secondary packaging for safe mailing
Choosing the correct container ensures specimen integrity, compliance with regulations, and reliable laboratory results.
Labeling Information
Submitting tick specimens for laboratory examination requires precise labeling to ensure accurate identification, pathogen detection, and traceability. Each label must contain standardized fields that enable researchers to assess epidemiological relevance and maintain chain‑of‑custody documentation.
Essential labeling elements include:
- Collection date (YYYY‑MM‑DD).
- Geographic coordinates or a detailed description of the site (e.g., town, county, GPS latitude/longitude).
- Host information (species, life stage of the host, health status).
- Tick species or provisional identification, if known.
- Life stage of the tick (larva, nymph, adult) and sex for adults.
- Collector’s name and contact details for follow‑up queries.
- Preservation method (e.g., ethanol concentration, frozen, dry).
- Unique specimen identifier (barcode or sequential number) linked to a database record.
Organizations that accept tick submissions for pathogen testing, species confirmation, or surveillance include:
- State and provincial public health laboratories, typically reachable through their vector‑borne disease divisions.
- Federal agencies such as the Centers for Disease Control and Prevention (CDC) Arbovirus Reference Laboratory and the National Institutes of Health (NIH) vector research centers.
- University entomology departments with accredited diagnostic facilities.
- Commercial diagnostic companies offering tick‑borne disease panels.
- Non‑governmental research consortia that operate regional tick surveillance networks.
Before dispatch, attach a waterproof label or sealed data sheet containing the required fields to each specimen container. Verify that the label format complies with the receiving laboratory’s submission guidelines; many institutions provide templates on their websites. Accurate labeling reduces processing delays, minimizes the risk of misidentification, and supports reliable public health reporting.
Required Information for Submission
Date and Location of Bite
When a tick is removed, the submission package must contain the exact date of the bite and a precise description of where the encounter occurred. This information enables the receiving laboratory to determine the tick species, assess the likelihood of pathogen presence, and contribute to regional surveillance data.
Accurate recording includes:
- Calendar date (day, month, year) of the bite.
- Geographic details: town or city, county, state, and, when possible, GPS coordinates or a detailed address.
- Habitat description (e.g., backyard garden, forest trail, pet’s bedding).
Providing these data allows analysts to:
- Match the specimen to known distribution maps for tick species.
- Correlate infection rates with seasonal activity patterns.
- Inform public‑health agencies about emerging risk zones.
Ticks can be sent to the following types of facilities:
- State or provincial health department laboratories.
- Federal agencies such as the Centers for Disease Control and Prevention.
- University entomology or vector‑biology departments.
- Veterinary diagnostic centers that accept wildlife samples.
- Commercial entities specializing in tick testing and pathogen detection.
Host Information
Host information provides essential context for any tick submitted for examination. Accurate data about the animal or human from which the tick was collected allows laboratories to interpret pathogen findings, assess epidemiological risk, and generate reliable reports.
Key elements of host data include:
- Species (common and scientific name)
- Age or life stage
- Sex, if determinable
- Health status (symptomatic, asymptomatic, known illnesses)
- Geographic coordinates or precise location description
- Date of collection
- Environmental conditions (temperature, habitat type)
Submission venues for tick analysis accept the above information alongside the specimen. Options are:
- State public health laboratories (e.g., CDC-affiliated state labs)
- University entomology or veterinary diagnostic centers
- Commercial diagnostic firms specializing in arthropod testing
- Online portals operated by national disease surveillance programs, which provide electronic forms for host data entry and specimen shipment instructions
Each venue requires a standardized data sheet or electronic entry. Attach the completed form to the package, label the specimen with a unique identifier, and use a temperature‑controlled container. Follow the specific courier guidelines indicated by the receiving laboratory to preserve sample integrity.
Symptoms Experienced
Ticks can transmit pathogens that manifest as localized skin irritation, fever, or systemic illness. Early identification of these signs is essential for timely treatment and accurate diagnosis.
Typical manifestations after a tick encounter include:
- Red, expanding rash at the bite site, often with a central clearing (erythema migrans).
- Flu‑like symptoms such as fever, chills, headache, and muscle aches.
- Joint pain or swelling, particularly in large joints.
- Neurological signs, including facial palsy, numbness, or difficulty concentrating.
- Gastrointestinal upset, nausea, or loss of appetite.
To confirm the presence of tick‑borne agents, the specimen must be sent to a qualified facility. Submission options are:
- State or regional public health laboratories that accept mailed tick samples.
- Veterinary diagnostic centers that process both animal and human tick specimens.
- Commercial reference laboratories offering online ordering and prepaid shipping kits.
- Academic research institutions with dedicated vector‑borne disease programs.
- Local health department clinics that provide drop‑off or courier services.
When submitting, include a detailed description of the observed symptoms, the date of the bite, and any relevant travel or exposure history. Accurate documentation enhances laboratory interpretation and supports appropriate public health response.
What Happens After Submission
Laboratory Procedures
DNA Extraction and PCR Testing
DNA extraction from ticks isolates genomic material necessary for polymerase chain reaction (PCR) assays that detect bacterial, viral, or protozoan agents. The extraction step must remove inhibitors, preserve nucleic acid integrity, and yield sufficient quantity for multiplex or single‑target PCR protocols. Commercial kits employing silica‑membrane or magnetic‑bead technology provide reproducible yields and are compatible with downstream amplification.
Submitting ticks for analysis requires facilities equipped with validated extraction workflows and certified PCR platforms. Options include:
- State or regional public health laboratories; they operate under regulatory oversight, offer pathogen panels for tick‑borne diseases, and provide results to clinicians and epidemiologists.
- University veterinary or microbiology departments; these institutions run research‑grade extraction and real‑time PCR assays, often accepting external specimens for fee‑based services.
- Commercial diagnostic companies specializing in arthropod testing; they deliver rapid turnaround, standardized reporting, and optional sequencing of PCR amplicons.
- Veterinary diagnostic laboratories; they focus on animal health, offering panels for common tick‑borne pathogens in livestock and companion animals.
- Research institutes with vector‑biology cores; they provide advanced methods such as quantitative PCR, digital droplet PCR, and multiplex panels, typically for collaborative projects.
Each venue requires proper specimen packaging, temperature control, and accompanying submission forms that specify target pathogens, collection date, and geographic origin. Selecting a laboratory aligns with the intended diagnostic scope, turnaround time, and regulatory requirements.
Visual Identification of Species
Visual identification of tick species relies on external morphology observable under magnification. Key characters include scutum shape, festoon count, mouthpart configuration, and leg segmentation. Accurate assessment requires calibrated microscopes, reference atlases, and, when needed, comparison with voucher specimens.
Specimens can be directed to the following institutions for expert analysis:
- State public‑health laboratories that operate vector‑borne disease programs.
- Federal agencies such as the Centers for Disease Control and Prevention, which maintain specialized entomology divisions.
- University entomology departments offering diagnostic services for researchers and the public.
- Accredited private diagnostic laboratories that provide species confirmation and pathogen testing.
- Museum collections that accept research material for taxonomic verification.
- Citizen‑science portals that forward submissions to qualified experts, for example, online tick‑identification networks.
Submission protocols require specimens to be preserved in ethanol (70 %–95 %) or frozen, placed in sealed containers, and accompanied by collection data: date, location (coordinates if available), host information, and any observed symptoms. High‑resolution photographs of dorsal and ventral views enhance the identification process and should be attached when possible.
Providing ticks for expert review yields definitive species determination, facilitates downstream molecular assays, and contributes to regional surveillance databases that inform public‑health interventions.
Interpreting Results
Understanding Positive and Negative Findings
Submitting tick specimens to qualified laboratories yields definitive diagnostic outcomes that guide public‑health responses and individual treatment decisions. Positive findings indicate the presence of pathogenic agents such as Borrelia, Rickettsia, or Anaplasma, confirming infection risk and prompting immediate clinical action. Negative findings demonstrate the absence of detectable pathogens, supporting reassurance and informing surveillance data without implying zero exposure risk.
Laboratories employ standardized assays—polymerase chain reaction, immunofluorescence, and culture—to generate results. A positive report includes organism identification, genetic markers, and, when available, antimicrobial susceptibility. A negative report lists the tests performed, detection limits, and any caveats regarding sample quality or timing. Both report types are archived in national databases to monitor geographic trends and emerging threats.
Common submission venues include:
- State health department vector‑borne disease units
- University‑affiliated entomology or microbiology laboratories
- Certified commercial diagnostic companies specializing in arthropod testing
- Federal agencies such as the CDC’s Arbovirus Surveillance Program
- Regional veterinary diagnostic centers for animal‑origin ticks
Choosing a recognized facility ensures accurate interpretation of positive and negative results, facilitating timely public‑health interventions and reliable epidemiological records.
Consulting with Healthcare Professionals
Consulting a qualified healthcare professional is essential when determining the appropriate venue for tick analysis. Physicians, dermatologists, and infectious‑disease specialists can assess exposure risk, recommend testing, and interpret results.
Typical destinations for tick specimens include:
- Hospital or university medical laboratories accredited for vector‑borne disease testing.
- State or regional public‑health laboratories that offer free or low‑cost analysis.
- Private veterinary or human diagnostic labs that provide rapid turnaround and detailed pathogen identification.
- Community health clinics that collaborate with entomology programs for sample submission.
The consulting clinician will:
- Verify that the tick is intact and properly preserved.
- Advise on required documentation, such as patient history and travel details.
- Select the most suitable laboratory based on geographic location, pathogen prevalence, and turnaround time.
- Explain the implications of potential findings and outline follow‑up treatment if necessary.
Engaging a healthcare professional streamlines the submission process, ensures compliance with laboratory protocols, and facilitates accurate diagnosis and timely medical response.
Follow-Up Actions
Treatment Protocols
Accurate diagnosis of tick‑borne illnesses begins with submitting collected specimens to qualified facilities. Reliable identification of species and pathogen testing enable clinicians to select evidence‑based treatment regimens.
Typical submission avenues include:
- State or provincial public health laboratories that offer free or subsidized testing.
- University veterinary or entomology departments with dedicated tick‑analysis programs.
- The Centers for Disease Control and Prevention’s Tick Testing Program, which processes mailed specimens nationwide.
- Commercial diagnostic laboratories that accept online orders and provide rapid results.
- Specialized mail‑in services that follow strict preservation protocols for DNA and serology assays.
Once a pathogen is confirmed, standard therapeutic approaches are applied:
- Bacterial infections (e.g., Lyme disease, Rocky Mountain spotted fever): doxycycline 100 mg twice daily for 10–21 days, adjusted for age and weight.
- Anaplasmosis and Ehrlichiosis: doxycycline 100 mg twice daily for 7–14 days; alternative agents for pregnant patients.
- Babesiosis: atovaquone 750 mg daily plus azithromycin 500 mg daily for 7–10 days; severe cases require clindamycin plus quinine.
- Viral encephalitis (e.g., Powassan virus): supportive care, hydration, and monitoring; no specific antivirals are approved.
- Tick‑induced allergic reactions: antihistamines and corticosteroids as indicated, with observation for anaphylaxis.
Prompt specimen submission and adherence to these protocols reduce morbidity and facilitate public‑health surveillance of emerging tick‑borne threats.
Preventive Measures
Preventive measures reduce the likelihood that ticks will need to be examined and ensure that any specimens submitted are representative of the environment in which they were collected.
- Wear long sleeves and trousers treated with permethrin when entering tick‑infested habitats.
- Apply EPA‑registered repellents containing DEET or picaridin to exposed skin.
- Conduct full‑body inspections each evening; remove attached ticks within 24 hours using fine‑pointed tweezers, grasping close to the skin and pulling straight upward.
- Keep yards trimmed, remove leaf litter, and create a barrier of wood chips or gravel between lawn and forested areas to limit tick migration.
- Use acaricide treatments on domestic animals and in high‑risk zones, following label instructions for dosage and frequency.
When a tick is collected for laboratory evaluation, follow these handling protocols to preserve diagnostic integrity:
- Place the specimen in a sealed, low‑temperature container (e.g., a screw‑cap tube with ethanol at 70 % or a dry, ventilated vial).
- Label the container with collection date, geographic coordinates, habitat description, and host information if known.
- Store the sample at 4 °C until shipment; avoid freezing, which can damage DNA.
- Dispatch the package to an accredited testing facility using a courier service that guarantees rapid delivery and complies with biological specimen regulations.
Adhering to these preventive and handling practices minimizes exposure risk, improves the quality of submitted specimens, and supports accurate diagnostic outcomes.