Where should a test for Demodex mites be submitted?

Where should a test for Demodex mites be submitted? - briefly

Submit the specimen to a dermatopathology or parasitology reference laboratory that offers microscopic analysis for Demodex. These facilities provide validated identification and reporting of mite presence.

Where should a test for Demodex mites be submitted? - in detail

Samples intended for identification of Demodex spp. must be processed by laboratories equipped for microscopic or molecular detection of these ectoparasites. The most reliable destinations include:

  • Dermatology or ophthalmology clinics that maintain in‑house parasitology sections; they can perform direct skin‑surface scrapings, eyelash examinations, or confocal microscopy.
  • Accredited clinical microbiology or parasitology reference laboratories (e.g., CDC‑approved facilities, state public health labs, or commercial entities such as Quest Diagnostics and LabCorp) that offer specialized mite analysis.
  • Veterinary diagnostic centers when the specimen originates from an animal host; these labs routinely handle Demodex canis, Demodex cati, etc.

Before dispatch, follow these procedural steps:

  1. Collect material using sterile curettes, adhesive tape, or eyelash plucking, depending on the site of infestation.
  2. Preserve specimens in 70 % ethanol or a suitable fixative; avoid formalin, which hinders mite morphology.
  3. Label each vial with patient identifier, collection date, anatomical location, and clinical indication.
  4. Complete the laboratory’s requisition form, specifying the desired method (light microscopy, scanning electron microscopy, or PCR‑based identification).
  5. Ship the package via overnight courier, maintaining temperature between 4 °C and 25 °C and complying with biohazard regulations.

Choosing a laboratory with CLIA certification or equivalent accreditation guarantees adherence to quality standards, accurate reporting, and appropriate interpretation of results. If the clinical setting lacks an in‑house service, referral to a recognized reference lab ensures definitive diagnosis and informs subsequent therapeutic decisions.