How should you correctly submit a scraping sample for a tick? - briefly
Submit the scraping sample as a single, high‑resolution PNG file labeled with the tick identifier and collection date, and upload it through the required submission portal. Ensure the file complies with size limits and includes a concise metadata sheet specifying species, location, and sampling method.
How should you correctly submit a scraping sample for a tick? - in detail
Proper submission of a scraping sample for a tick requires adherence to a defined sequence of actions to ensure data integrity and regulatory compliance.
The process begins with verification of the tick’s identification. Confirm species, developmental stage, and collection location using a validated taxonomic key. Record this information in a standardized log, including date and collector’s identifier.
Next, prepare the sample according to the following specifications:
- Use a sterile scalpel or fine‑tipped instrument to obtain tissue from the ventral surface, avoiding contamination from external debris.
- Place the collected material into a pre‑labeled microcentrifuge tube containing 95 % ethanol, ensuring a tissue‑to‑solvent ratio of at least 1 : 10.
- Seal the tube securely, then store at –20 °C until shipment.
Documentation must accompany the physical sample. Required items include:
- Completed submission form with fields for taxonomic details, geographic coordinates, and collection metadata.
- Photographic evidence of the tick, captured with a scale bar and background contrast, formatted as JPEG or PNG.
- Chain‑of‑custody statement signed by the collector and any subsequent handlers.
Submission itself follows these steps:
- Package the sealed tube in a secondary container with absorbent material to mitigate leakage.
- Include the documentation packet in a waterproof envelope placed inside the same outer package.
- Dispatch via a courier service that offers temperature‑controlled transport and tracking.
- Retain the tracking number and confirm receipt by the receiving laboratory within 48 hours.
Upon arrival, the receiving facility will perform the following verification actions:
- Inspect the external packaging for integrity and temperature log compliance.
- Cross‑check the sample label against the accompanying metadata.
- Conduct a preliminary microscopic examination to confirm the presence of tick tissue.
If discrepancies arise, the facility will issue a rejection notice detailing the specific deficiency, prompting resubmission with corrected materials.
Common errors to avoid include:
- Using non‑sterile tools that introduce microbial contaminants.
- Insufficient ethanol concentration, leading to DNA degradation.
- Omitting geographic coordinates or providing inaccurate GPS data.
Adhering strictly to the outlined protocol maximizes the likelihood of successful analysis and reduces turnaround time for downstream applications.