How can you tell a female bedbug from a male?

How can you tell a female bedbug from a male? - briefly

Females have a broader, rounded abdomen that lacks a terminal tip, while males possess a narrower, tapered abdomen ending in a visible genital capsule. This morphological difference is the quickest way to distinguish the sexes.

How can you tell a female bedbug from a male? - in detail

Bedbugs (Cimex lectularius) can be sexed only after they reach the adult stage. The differences are confined to the posterior abdomen and require magnification of at least 30×.

Key external characteristics

  • Abdominal shape – Females have a broader, more rounded abdomen that tapers to a pointed ovipositor. Males possess a slimmer abdomen that ends in a slightly concave outline.
  • Terminal segment – In females the last abdominal segment bears a visible ovipositor sheath, a small, needle‑like extension used for egg deposition. In males the same region shows the aedeagus, a tubular structure that may appear as a tiny protrusion or a faint groove.
  • SizeAdult females are typically 5–6 mm long, while males average 4.5–5 mm. The size gap is modest and should not be the sole criterion.
  • Coloration – Both sexes share the same reddish‑brown hue; no reliable color differences exist.

Procedure for accurate determination

  1. Collect a live adult and immobilize it by placing the specimen in a freezer for 10–15 minutes or by using CO₂.
  2. Place the bug on a clear slide with a drop of ethanol or water to flatten the abdomen gently.
  3. Observe under a stereomicroscope focusing on the terminal abdominal segments.
  4. Identify the ovipositor (female) or the aedeagus (male) based on shape and orientation.
  5. Record the measurement of overall body length to support the visual assessment.

Additional notes

  • Nymphs cannot be sexed because they lack developed genital structures.
  • Dissection of the abdomen provides definitive confirmation but is rarely required for routine identification.
  • Accurate sexing is essential for studies of population dynamics, reproductive rates, and control strategies.