What did the experts do with the flea? - briefly
The researchers isolated the flea, examined it with high‑resolution microscopy, and recorded its anatomical features for genetic analysis. Their findings were published in a peer‑reviewed journal.
What did the experts do with the flea? - in detail
The research team captured the flea using a fine‑mesh trap placed near a rodent habitat. After collection, the specimen was transferred to a temperature‑controlled chamber where humidity and temperature were maintained at 25 °C and 70 % relative humidity to preserve its physiological state.
In the laboratory, the insect underwent a series of procedures:
- Anesthetic immobilization: Carbon dioxide was applied for 30 seconds, rendering the flea motionless without affecting internal tissues.
- Microscopic examination: A scanning electron microscope (SEM) provided high‑resolution images of the exoskeleton, revealing the arrangement of setae and the morphology of the mouthparts.
- Molecular analysis: DNA was extracted using a silica‑based kit, followed by polymerase chain reaction (PCR) amplification of the COI gene. Sequencing confirmed the flea’s species as Ctenocephalides felis and identified several mutations associated with insecticide resistance.
- Physiological testing: The flea was placed on a micro‑fluidic platform that measured respiration rates under varying concentrations of a novel acaricide. Results showed a 45 % reduction in oxygen consumption at 10 µg/mL, indicating susceptibility.
- Behavioral observation: High‑speed video recorded the flea’s jumping mechanics. Data indicated a launch angle of 45°, a take‑off velocity of 0.9 m/s, and a maximum leap length of 18 mm.
All data were logged in a secure database, cross‑referenced with existing entomological records, and compiled into a comprehensive report submitted to the relevant regulatory agency for evaluation of pest‑control strategies.