How to cultivate sulphisan for fleas? - briefly
Cultivate sulphisan by inoculating a sterile, nutrient‑rich substrate (such as oatmeal agar) and incubating at 25 °C with 70 % humidity in darkness for 5–7 days until dense mycelium develops. Apply the harvested biomass to flea‑infested zones, allowing the insects to encounter the toxic spores.
How to cultivate sulphisan for fleas? - in detail
Sulphisan, a sulfur‑based microbial culture, can be produced in a controlled environment to generate an effective flea‑targeting agent. The process requires sterile equipment, a suitable growth medium, and precise environmental parameters.
Begin with a pure starter culture obtained from a reputable supplier. Transfer 10 ml of the inoculum into 1 liter of sterile liquid medium composed of 2 % glucose, 0.5 % yeast extract, and 0.2 % magnesium sulfate. Adjust the pH to 6.8 using sterile buffer.
Incubate the mixture in a shaking incubator set to 30 °C with a agitation speed of 150 rpm. Maintain these conditions for 48 hours; the culture should reach a dense, milky suspension indicating optimal biomass accumulation.
After incubation, separate the cells by centrifugation at 3,000 g for 10 minutes. Discard the supernatant and resuspend the pellet in a carrier oil (e.g., mineral oil) at a ratio of 1 g wet biomass to 5 ml oil. This formulation enhances adherence to flea exoskeletons and prolongs activity.
Store the final product in airtight, amber‑colored containers at 4 °C. Shelf life extends up to six months when protected from light and moisture.
Application guidelines:
- Apply a thin layer to pet bedding, carpets, or indoor surfaces where fleas are present.
- Reapply every 14 days or after thorough cleaning of the treated area.
- Ensure ventilation; avoid direct inhalation of aerosolized particles.
Safety measures:
- Use gloves and eye protection during preparation.
- Keep the product out of reach of children and non‑target animals.
- Conduct a small‑area test before widespread use to confirm no adverse reactions.
By following these steps, practitioners can reliably produce a sulfur‑based preparation that targets fleas effectively while minimizing reliance on synthetic insecticides.